A method to determine and simulate the permeation through a gel matrix in a multi-organ-chip

The Multi-Organ-Chip (MOC) is a new micro size bioreactor. It offers two tissue cultivation segments (e.g. skin model, liver cells), which are connected by a dynamic micro pump system. [1,2] One future application of this system is the dermal application of substances for drug testing in vitro. In this study a method to simulate and validate the permeation of fluorescein sodium salt through a barrier in the MOC was established. Fluorescein sodium salt has a molecular weight of 376.28 g/mol which is in the range of steroid hormones like testosterone (288.4 g/mol), aldesterone (360.4 g/mol), hydrocortisone (362.5.4 g/mol) and corticosterone (346 g/mol) [3]. Furthermore, first permeation experiments through four cell-collagen models, as reference for skin models, were carried out.