Functional Complementation Assay for 47 MUTYH Variants in a MutY-Disrupted Escherichia Coli Strain

ABSTRACT MUTYH‐associated polyposis (MAP) is an adenomatous polyposis transmitted in an autosomal‐recessive pattern, involving biallelic inactivation of the MUTYH gene. Loss of a functional MUTYH protein will result in the accumulation of G:T mismatched DNA caused by oxidative damage. Although p.Y179C and p.G396D are the two most prevalent MUTYH variants, more than 200 missense variants have been detected. It is difficult to determine whether these variants are disease‐causing mutations or single‐nucleotide polymorphisms. To understand the functional consequences of these variants, we generated 47 MUTYH gene variants via site‐directed mutagenesis, expressed the encoded proteins in MutY‐disrupted Escherichia coli, and assessed their abilities to complement the functional deficiency in the E. coli by monitoring spontaneous mutation rates. Although the majority of variants exhibited intermediate complementation relative to the wild type, some variants severely interfered with this complementation. However, some variants retained functioning similar to the wild type. In silico predictions of functional effects demonstrated a good correlation. Structural prediction of MUTYH based on the MutY protein structure allowed us to interpret effects on the protein stability or catalytic activity. These data will be useful for evaluating the functional consequences of missense MUTYH variants detected in patients with suspected MAP. We examined 47 MUTYH variants in using functional complementation assay with MutY‐disrupted E. coli. Based on the BER activities, we evaluated 17 variants functionally retained (green), 17 variants partially defective (yellow) and 13 variants functionally defective (red). Our mapping of variants on the predicted MUTYH structure based on homology modeling indicated that most of the variants that were predicted to be situated around the DNA‐binding site and the [4Fe‐4S] clustering pocket were found to be functionally defective variants.