Whole-transcriptome gene expression profiling in an epidermolysis bullosa simplex Dowling-Meara model keratinocyte cell line uncovered novel, potential therapeutic targets and affected pathways

Whole-transcriptome gene expression profiling in an epidermolysis bullosa simplex Dowling-Meara model keratinocyte cell line uncovered novel, potential therapeutic targets and affected pathways

To be able to develop effective therapeutics for epidermolysis bullosa simplex (EBS), it is necessary to elucidate the molecular pathomechanisms that give rise to the disease's characteristic severe skin-blistering phenotype. Starting with a whole-transcriptome microarray analysis of an EBS Dow...

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Veröffentlicht in:BMC research notes 2015-12, Vol.8 (1), p.785-785, Article 785
Hauptverfasser: Herzog, Julia, Rid, Raphaela, Wagner, Martin, Hundsberger, Harald, Eger, Andreas, Bauer, Johann, Önder, Kamil
Format: Artikel
Sprache:eng
Schlagworte:
Analysis
Blotting, Western
Cell Line
DNA microarrays
Epidermolysis bullosa
Epidermolysis Bullosa Simplex - drug therapy
Epidermolysis Bullosa Simplex - genetics
Epidermolysis Bullosa Simplex - pathology
Gene expression
Gene Expression Profiling - methods
Gene Ontology
Genes
Genetic Predisposition to Disease - genetics
Health aspects
Homeopathy
Humans
Keratin
Keratinocytes - drug effects
Keratinocytes - metabolism
Materia medica and therapeutics
Molecular Targeted Therapy - methods
Mutation
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction - drug effects
Signal Transduction - genetics
Skin
Therapeutics
Transcriptome - genetics
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Beschreibung
Zusammenfassung:To be able to develop effective therapeutics for epidermolysis bullosa simplex (EBS), it is necessary to elucidate the molecular pathomechanisms that give rise to the disease's characteristic severe skin-blistering phenotype. Starting with a whole-transcriptome microarray analysis of an EBS Dowling-Meara model cell line (KEB7), we identified 207 genes showing differential expression relative to control keratinocytes. A complementary qRT-PCR study of 156 candidates confirmed 76.58 % of the selected genes to be significantly up-regulated or down-regulated (p-value
ISSN:1756-0500
1756-0500
DOI:10.1186/s13104-015-1783-7