Isolation and Functional Analysis of Human Neutrophils

Isolation and Functional Analysis of Human Neutrophils

This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll‐Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described. © 2015 b...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Current Protocols in Immunology 2015-11, Vol.111 (1), p.7.23.1-7.23.16
Hauptverfasser: Kuhns, Douglas B., Priel, Debra A. Long, Chu, Jessica, Zarember, Kol A.
Format: Artikel
Sprache:eng
Schlagworte:
Centrifugation, Density Gradient - methods
Cytochromes c - metabolism
Dextrans
Ficoll
Flow Cytometry - methods
Humans
Hydrogen Peroxide - analysis
Hydrogen Peroxide - metabolism
innate immunity
leukocyte
neutrophil
Neutrophils - cytology
Neutrophils - immunology
NOX2
phagocyte
Phagocytosis - immunology
Povidone
Respiratory Burst - physiology
Silicon Dioxide
Staphylococcus aureus - immunology
Superoxide Dismutase - metabolism
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll‐Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described. © 2015 by John Wiley & Sons, Inc.
ISSN:1934-3671
1934-368X
DOI:10.1002/0471142735.im0723s111