Surface vs. solution hybridization: effects of salt, temperature, and probe type

Surface vs. solution hybridization: effects of salt, temperature, and probe type

Hybridization thermodynamics on solid supports are compared with those in solution for two types of hybridization probe, DNA and uncharged morpholino oligonucleotides of identical sequences. Trends in hybridization affinity are discussed with respect to ionic strength, temperature, and surface behav...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2015-12, Vol.51 (97), p.17245-17248
Hauptverfasser: Qiao, Wanqiong, Chiang, Hao-Chun, Xie, Hui, Levicky, Rastislav
Format: Artikel
Sprache:eng
Schlagworte:
Affinity
Deoxyribonucleic acid
Gene sequencing
Molecular Probes
Oligonucleotides
Salts - chemistry
Solutions - chemistry
Strength
Surface Properties
Temperature
Thermodynamics
Trends
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Beschreibung
Zusammenfassung:Hybridization thermodynamics on solid supports are compared with those in solution for two types of hybridization probe, DNA and uncharged morpholino oligonucleotides of identical sequences. Trends in hybridization affinity are discussed with respect to ionic strength, temperature, and surface behavior.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc06674c