Effect of manganese on heat stress protein synthesis of new—born rats

To study the effect of manganese (Mn) on heat stress protein 70 (HSP70) synthesis in the brain and liver of new-born rats whose mother-rats were exposed to Mn. 32 female rats were randomly divided into four groups. One group was administrated with physiological saline only as control group, the other three groups were administrated with 7.5, 15 and 30 mg x kg(-1) manganese chloride (MnCl2) by intraperitioneal injection every two days for two weeks. After delivery, the mother-rats received MnCl2 unceasingly for a week with the same method. Then the contents of Mn Zn Cu and Fe in the livers of the new-born rats were determined by atomic absorption spectroscopy; The level of HSP70 in the brains and the livers of the new-born rats as detected by Western-dot-blotting, and the SOD activities were measured simultaneously. The contents of Mn in the livers of new-born rats of the experimental groups(respective 1.38+/-0.18, 2.73+/-0.65,3.44+/-0.89 microg x g(-1)) were significantly increased compared with the control group(0.88+/-0.18 microg x g(-1); P