Hyposmotic membrane stretch potentiated muscarinic receptor agonist—induced depolarization of membrane potential in guinea—pig gastric myocytes

AIM：To investigate the relationship between hyposmotic membrane stretch and muscarinic receptor agonist-induced depolarization of membrane potential in antral gastric circular myocytes of guinea-pig.METHODS:Using whole cell patch-clamp technique recorded membrane potential and current in single gast...

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Veröffentlicht in:	World journal of gastroenterology : WJG 2002-08, Vol.8 (4), p.724-727
Hauptverfasser:	Li, Lin, Jin, Nan-Ge, Piao, Lin, Hong, Ming-Yu, Jin, Zheng-Yuan, Li, Ying, Xu, Wen-Xie
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Basic Research Carbachol - pharmacology Cell Membrane - physiology Female Guinea Pigs In Vitro Techniques Male Membrane Potentials - drug effects Muscarinic Agonists - pharmacology Muscle Spindles - drug effects Muscle Spindles - physiology Muscle, Smooth - cytology Muscle, Smooth - drug effects Muscle, Smooth - physiology Osmotic Pressure Stomach - cytology Stomach - physiology 低渗性膈膜牵张动物实验去极现象毒蕈碱型胆碱受体胃肌细胞
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Zusammenfassung:	AIM：To investigate the relationship between hyposmotic membrane stretch and muscarinic receptor agonist-induced depolarization of membrane potential in antral gastric circular myocytes of guinea-pig.METHODS:Using whole cell patch-clamp technique recorded membrane potential and current in single gastric myocytes isolated by collagenase.RESULTS:Hyposmotic membrane stretchhyperpolarized membrane potential from-60.0mV±1.0mVto-67.9mV±1.0mV,TEA(10mmol/L),a nonselective potassium channel blocker significantly inhibitea hyposmotic membrane stretch-induced hyperpolarization.After KCI in the pipette and NaCl in the external solution were replaced by CsCI to block the potassium current,hyposmotic membrane stretch depolarized the membrane potential from-60.0mV±1.0mVto-44.8mV±2.3mV(P
ISSN:	1007-9327 2219-2840
DOI:	10.3748/wjg.v8.i4.724