Identification of novel, therapy-responsive protein biomarkers in a mouse model of Duchenne muscular dystrophy by aptamer-based serum proteomics

There is currently an urgent need for biomarkers that can be used to monitor the efficacy of experimental therapies for Duchenne Muscular Dystrophy (DMD) in clinical trials. Identification of novel protein biomarkers has been limited due to the massive complexity of the serum proteome and the presen...

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Veröffentlicht in:	Scientific reports 2015-11, Vol.5 (1), p.17014-17014, Article 17014
Hauptverfasser:	Coenen-Stass, Anna M. L., McClorey, Graham, Manzano, Raquel, Betts, Corinne A., Blain, Alison, Saleh, Amer F., Gait, Michael J., Lochmüller, Hanns, Wood, Matthew J. A., Roberts, Thomas C.
Format:	Artikel
Sprache:	eng
Schlagworte:	38/79 631/45 692/53/2423 82/80 ADAM Proteins - blood ADAM Proteins - genetics ADAMTS5 Protein Animals Aptamers, Nucleotide - pharmacology Biomarkers, Pharmacological - blood Blood Proteins - genetics Blood Proteins - metabolism Calcium-Calmodulin-Dependent Protein Kinase Type 2 - blood Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics Disease Models, Animal Dystrophin - agonists Dystrophin - deficiency Dystrophin - genetics Gene Expression Profiling Gene Expression Regulation Humanities and Social Sciences Humans Male Mice Mice, Inbred C57BL Mice, Inbred mdx multidisciplinary Muscular Dystrophy, Duchenne - genetics Muscular Dystrophy, Duchenne - metabolism Muscular Dystrophy, Duchenne - pathology Muscular Dystrophy, Duchenne - therapy Oligonucleotides, Antisense - pharmacology Phosphoric Monoester Hydrolases - blood Phosphoric Monoester Hydrolases - genetics Protein Kinases - blood Protein Kinases - genetics Protein Serine-Threonine Kinases Proteomics - methods Science
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Zusammenfassung:	There is currently an urgent need for biomarkers that can be used to monitor the efficacy of experimental therapies for Duchenne Muscular Dystrophy (DMD) in clinical trials. Identification of novel protein biomarkers has been limited due to the massive complexity of the serum proteome and the presence of a small number of very highly abundant proteins. Here we have utilised an aptamer-based proteomics approach to profile 1,129 proteins in the serum of wild-type and mdx (dystrophin deficient) mice. The serum levels of 96 proteins were found to be significantly altered ( P
ISSN:	2045-2322 2045-2322
DOI:	10.1038/srep17014