SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy

Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck , a suite of ImageJ plugins enabling users to iden...

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Veröffentlicht in:	Scientific reports 2015-11, Vol.5 (1), p.15915-15915, Article 15915
Hauptverfasser:	Ball, Graeme, Demmerle, Justin, Kaufmann, Rainer, Davis, Ilan, Dobbie, Ian M., Schermelleh, Lothar
Format:	Artikel
Sprache:	eng
Schlagworte:	14 631/114/1564 631/1647/328/2238 Algorithms Calibration Data processing Humanities and Social Sciences Illumination Image processing Image Processing, Computer-Assisted - methods Imaging, Three-Dimensional - methods Lighting - methods Microscopy Microscopy, Fluorescence - methods multidisciplinary Open source software Reproducibility of Results Science
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Beschreibung
Zusammenfassung:	Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck , a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms and will promote routine application of super-resolution SIM imaging in cell biology.
ISSN:	2045-2322 2045-2322
DOI:	10.1038/srep15915