A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also al...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Lab on a chip 2015-07, Vol.15 (14), p.2918-2922
Hauptverfasser:	Mozdzierz, Nicholas J, Love, Kerry R, Lee, Kevin S, Lee, Harry L T, Shah, Kartik A, Ram, Rajeev J, Love, J Christopher
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioreactors Biotechnology Cells, Cultured Cultivation Human Growth Hormone - biosynthesis Humans Interferon alpha-2 Interferon-alpha - biosynthesis Microfluidic Analytical Techniques - instrumentation Microfluidics Microorganisms Optimization Perfusion - instrumentation Pichia Pichia - cytology Pichia - metabolism Proteins Recombinant Proteins - biosynthesis Small scale
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing.
ISSN:	1473-0197 1473-0189
DOI:	10.1039/c5lc00443h