Analysis of serum IgE reactivity profiles with microarrayed allergens indicates absence of de novo IgE sensitizations in adults

To address the question of whether and how frequently adult allergic patients and nonallergic subjects have IgE sensitizations to new allergen molecules, we performed a retrospective analysis of serum samples using an allergen microarray that contained 85 different purified allergen molecules (ISAC; Phadia, Uppsala, Sweden).2 The 85 allergens on the chip represented 50 allergen families with experimentally confirmed cross-reactivity (see Fig E1 in this article's Online Repository at www.jacionline.org).