An electrochemiluminescence assay for analysis of rabies virus glycoprotein content in rabies vaccines

Highlights • An antigen-capture electrochemiluminescent (ECL) assay was developed. • The ECL assay was used to measure the antigen content of rabies vaccines. • The ECL assay using monoclonal antibody 2-21-14 was sensitive and specific. • Antigenicity was compared to immunogenicity in a mouse model....

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Veröffentlicht in:	Vaccine 2013-07, Vol.31 (33), p.3333-3338
Hauptverfasser:	Smith, Todd G, Ellison, James A, Ma, Xiaoyue, Kuzmina, Natalia, Carson, William C, Rupprecht, Charles E
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Animals Antibodies, Monoclonal - chemistry Antibodies, Neutralizing - blood Antibodies, Viral - blood Antibody Specificity Antigenicity Antigens Antigens, Viral - chemistry Antigens, Viral - isolation & purification Applied microbiology Biological and medical sciences Biosafety Cloning Confidence intervals Electrochemical Techniques Electrochemiluminescent assay Epitopes Female Fundamental and applied biological sciences. Psychology Glycoprotein glycoproteins Glycoproteins - chemistry Glycoproteins - isolation & purification humans immune response Immunization Immunogenicity Luminescent Measurements - methods Methods Mice Microbiology Miscellaneous monoclonal antibodies Multiple myeloma neutralization neutralizing antibodies Protein Folding Proteins Rabies Rabies - prevention & control Rabies lyssavirus Rabies Vaccines - chemistry Rabies virus Sensitivity and Specificity vaccination Vaccine Vaccine Potency Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Veterinary medicine Viral Envelope Proteins - chemistry Viral Envelope Proteins - isolation & purification Virology World Health Organization
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Beschreibung
Zusammenfassung:	Highlights • An antigen-capture electrochemiluminescent (ECL) assay was developed. • The ECL assay was used to measure the antigen content of rabies vaccines. • The ECL assay using monoclonal antibody 2-21-14 was sensitive and specific. • Antigenicity was compared to immunogenicity in a mouse model. • The ECL assay was able to distinguish between borderline vaccine lots.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2013.05.081