Time Course of Ca2+Concentration Triggering Exocytosis in Neuroendocrine Cells
We have used the secretory response of chromaffin cells to estimate the submembrane intracellular Ca2+concentration ([Ca2+]i) "seen" by secretory granules during short depolarizations. The rate of secretion during a depolarization was assessed by combining the electrochemical method of amp...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1994-12, Vol.91 (26), p.12765-12769 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Chow, Robert H. Klingauf, Jurgen Neher, Erwin |
| description | We have used the secretory response of chromaffin cells to estimate the submembrane intracellular Ca2+concentration ([Ca2+]i) "seen" by secretory granules during short depolarizations. The rate of secretion during a depolarization was assessed by combining the electrochemical method of amperometry and electrical capacitance measurements. The rate was then related to [Ca2+]ibased on a previous characterization of how Ca2+affects the dynamics of vesicle priming and fusion in chromaffin cells [Heinemann, C., Chow, R. H., Neher, E. \& Zucker, R. S. (1994) Biophys. J. 67, in press]. Calculated [Ca2+]irose during the depolarization to a peak of |
| doi_str_mv | 10.1073/pnas.91.26.12765 |
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The rate of secretion during a depolarization was assessed by combining the electrochemical method of amperometry and electrical capacitance measurements. The rate was then related to [Ca2+]ibased on a previous characterization of how Ca2+affects the dynamics of vesicle priming and fusion in chromaffin cells [Heinemann, C., Chow, R. H., Neher, E. \& Zucker, R. S. (1994) Biophys. J. 67, in press]. Calculated [Ca2+]irose during the depolarization to a peak of <10 μ M, then decayed over tens of milliseconds. In synapses, vesicles are presumed to be located within nanometers of Ca2+channels where [Ca2+]iis believed to rise in only microseconds to near steady-state levels of hundreds of micromolar. Channel closure should lead to a decrease in [Ca2+]ialso in microseconds. Our findings of the slower time course and the lower peak [Ca2+]isuggest that in chromaffin cells, unlike synapses, Ca2+channels and vesicles are not strictly colocalized. This idea is consistent with previously published data on dense-core vesicle secretion from diverse cell types.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.91.26.12765</identifier><identifier>PMID: 7809118</identifier><language>eng</language><publisher>United States: National Academy of the Sciences of the United States of America</publisher><subject>Action potentials ; Adrenal Medulla - metabolism ; Animals ; Calcium - metabolism ; Capacitance ; Cattle ; Chromaffin cells ; Depolarization ; Electrodes ; Exocytosis ; Histograms ; In Vitro Techniques ; Kinetics ; Membrane Fusion ; Membrane Potentials ; Neurons ; Secretion ; Synapses ; Time Factors</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1994-12, Vol.91 (26), p.12765-12769</ispartof><rights>Copyright 1994 The National Academy of Sciences of the United States of America</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-2147b7aa0925671380699a8000860cc430ab186d4d244ce296607f08e747add93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/91/26.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2366423$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2366423$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7809118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chow, Robert H.</creatorcontrib><creatorcontrib>Klingauf, Jurgen</creatorcontrib><creatorcontrib>Neher, Erwin</creatorcontrib><title>Time Course of Ca2+Concentration Triggering Exocytosis in Neuroendocrine Cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We have used the secretory response of chromaffin cells to estimate the submembrane intracellular Ca2+concentration ([Ca2+]i) "seen" by secretory granules during short depolarizations. The rate of secretion during a depolarization was assessed by combining the electrochemical method of amperometry and electrical capacitance measurements. The rate was then related to [Ca2+]ibased on a previous characterization of how Ca2+affects the dynamics of vesicle priming and fusion in chromaffin cells [Heinemann, C., Chow, R. H., Neher, E. \& Zucker, R. S. (1994) Biophys. J. 67, in press]. Calculated [Ca2+]irose during the depolarization to a peak of <10 μ M, then decayed over tens of milliseconds. In synapses, vesicles are presumed to be located within nanometers of Ca2+channels where [Ca2+]iis believed to rise in only microseconds to near steady-state levels of hundreds of micromolar. Channel closure should lead to a decrease in [Ca2+]ialso in microseconds. Our findings of the slower time course and the lower peak [Ca2+]isuggest that in chromaffin cells, unlike synapses, Ca2+channels and vesicles are not strictly colocalized. This idea is consistent with previously published data on dense-core vesicle secretion from diverse cell types.</description><subject>Action potentials</subject><subject>Adrenal Medulla - metabolism</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Capacitance</subject><subject>Cattle</subject><subject>Chromaffin cells</subject><subject>Depolarization</subject><subject>Electrodes</subject><subject>Exocytosis</subject><subject>Histograms</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Membrane Fusion</subject><subject>Membrane Potentials</subject><subject>Neurons</subject><subject>Secretion</subject><subject>Synapses</subject><subject>Time Factors</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1PGzEQxa0KBIH23gMVe6oqoU3HXq8_pF6qFQWkKFzSs-V4vanRxk7tXQT_fR2SRnDh5MP7vTczfgh9xjDFwKvvG6_TVOIpYVNMOKs_oAkGiUtGJRyhCQDhpaCEnqKzlB4AQNYCTtAJF5nCYoLmC7e2RRPGmGwRuqLR5KoJ3lg_RD244ItFdKuVjc6viuunYJ6HkFwqnC_mdozB-jaYLOYM2_fpIzrudJ_sp_17jn7_ul40t-Xs_uau-TkrDWX1UBJM-ZJrDZLUjONKAJNSi7yfYGAMrUAvsWAtbQmlxhLJGPAOhOWU67aV1Tn6scvdjMu1bXfr9moT3VrHZxW0U28V7_6oVXhUtK4JZPvXvT2Gv6NNg1q7ZPIB2tswJsWZxALIdg7sQBNDStF2hxEY1LYBtW1ASawIUy8NZMuX16sdDPsvz_q3vb51_ldfJahu7PvBPg0ZvXwfzcTFjnhIQ4gHhFSMUVJV_wA3_aQm</recordid><startdate>19941220</startdate><enddate>19941220</enddate><creator>Chow, Robert H.</creator><creator>Klingauf, Jurgen</creator><creator>Neher, Erwin</creator><general>National Academy of the Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19941220</creationdate><title>Time Course of Ca2+Concentration Triggering Exocytosis in Neuroendocrine Cells</title><author>Chow, Robert H. ; Klingauf, Jurgen ; Neher, Erwin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-2147b7aa0925671380699a8000860cc430ab186d4d244ce296607f08e747add93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Action potentials</topic><topic>Adrenal Medulla - metabolism</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Capacitance</topic><topic>Cattle</topic><topic>Chromaffin cells</topic><topic>Depolarization</topic><topic>Electrodes</topic><topic>Exocytosis</topic><topic>Histograms</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Membrane Fusion</topic><topic>Membrane Potentials</topic><topic>Neurons</topic><topic>Secretion</topic><topic>Synapses</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chow, Robert H.</creatorcontrib><creatorcontrib>Klingauf, Jurgen</creatorcontrib><creatorcontrib>Neher, Erwin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chow, Robert H.</au><au>Klingauf, Jurgen</au><au>Neher, Erwin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Time Course of Ca2+Concentration Triggering Exocytosis in Neuroendocrine Cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1994-12-20</date><risdate>1994</risdate><volume>91</volume><issue>26</issue><spage>12765</spage><epage>12769</epage><pages>12765-12769</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>We have used the secretory response of chromaffin cells to estimate the submembrane intracellular Ca2+concentration ([Ca2+]i) "seen" by secretory granules during short depolarizations. The rate of secretion during a depolarization was assessed by combining the electrochemical method of amperometry and electrical capacitance measurements. The rate was then related to [Ca2+]ibased on a previous characterization of how Ca2+affects the dynamics of vesicle priming and fusion in chromaffin cells [Heinemann, C., Chow, R. H., Neher, E. \& Zucker, R. S. (1994) Biophys. J. 67, in press]. Calculated [Ca2+]irose during the depolarization to a peak of <10 μ M, then decayed over tens of milliseconds. In synapses, vesicles are presumed to be located within nanometers of Ca2+channels where [Ca2+]iis believed to rise in only microseconds to near steady-state levels of hundreds of micromolar. Channel closure should lead to a decrease in [Ca2+]ialso in microseconds. Our findings of the slower time course and the lower peak [Ca2+]isuggest that in chromaffin cells, unlike synapses, Ca2+channels and vesicles are not strictly colocalized. This idea is consistent with previously published data on dense-core vesicle secretion from diverse cell types.</abstract><cop>United States</cop><pub>National Academy of the Sciences of the United States of America</pub><pmid>7809118</pmid><doi>10.1073/pnas.91.26.12765</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Action potentials Adrenal Medulla - metabolism Animals Calcium - metabolism Capacitance Cattle Chromaffin cells Depolarization Electrodes Exocytosis Histograms In Vitro Techniques Kinetics Membrane Fusion Membrane Potentials Neurons Secretion Synapses Time Factors |
| title | Time Course of Ca2+Concentration Triggering Exocytosis in Neuroendocrine Cells |
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