Time Course of Ca2+Concentration Triggering Exocytosis in Neuroendocrine Cells

Time Course of Ca2+Concentration Triggering Exocytosis in Neuroendocrine Cells

We have used the secretory response of chromaffin cells to estimate the submembrane intracellular Ca2+concentration ([Ca2+]i) "seen" by secretory granules during short depolarizations. The rate of secretion during a depolarization was assessed by combining the electrochemical method of amp...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1994-12, Vol.91 (26), p.12765-12769
Hauptverfasser: Chow, Robert H., Klingauf, Jurgen, Neher, Erwin
Format: Artikel
Sprache:eng
Schlagworte:
Action potentials
Adrenal Medulla - metabolism
Animals
Calcium - metabolism
Capacitance
Cattle
Chromaffin cells
Depolarization
Electrodes
Exocytosis
Histograms
In Vitro Techniques
Kinetics
Membrane Fusion
Membrane Potentials
Neurons
Secretion
Synapses
Time Factors
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Zusammenfassung:We have used the secretory response of chromaffin cells to estimate the submembrane intracellular Ca2+concentration ([Ca2+]i) "seen" by secretory granules during short depolarizations. The rate of secretion during a depolarization was assessed by combining the electrochemical method of amperometry and electrical capacitance measurements. The rate was then related to [Ca2+]ibased on a previous characterization of how Ca2+affects the dynamics of vesicle priming and fusion in chromaffin cells [Heinemann, C., Chow, R. H., Neher, E. \& Zucker, R. S. (1994) Biophys. J. 67, in press]. Calculated [Ca2+]irose during the depolarization to a peak of
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.91.26.12765