Discrimination between native and Tn6010-associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by using a two-step strategy

Discrimination between native and Tn6010-associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by using a two-step strategy

We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella s...

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Veröffentlicht in:Antimicrobial agents and chemotherapy 2015-09, Vol.59 (9), p.5838-5840
Hauptverfasser: Guillard, Thomas, Lebreil, Anne-Laure, Hansen, Lars Hestbjerg, Kisserli, Aymric, Berger, Sibel, Lozniewski, Alain, Alauzet, Corentine, de Champs, Christophe
Format: Artikel
Sprache:eng
Schlagworte:
Anti-Bacterial Agents
Anti-Bacterial Agents - pharmacology
Enterobacteriaceae
Enterobacteriaceae - drug effects
Klebsiella
Klebsiella - drug effects
Mechanisms of Resistance
Microbial Sensitivity Tests
Polymerase Chain Reaction
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Zusammenfassung:We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.00669-15