Fungal endophytes of Vanilla planifolia across Réunion Island: isolation, distribution and biotransformation
The objective of the work was to characterize fungal endophytes from aerial parts of Vanilla planifolia. Also, to establish their biotransformation abilities of flavor-related metabolites. This was done in order to find a potential role of endophytes on vanilla flavors. Twenty three MOTUs were obtai...
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| Veröffentlicht in: | BMC plant biology 2015-06, Vol.15 (1), p.142-142, Article 142 |
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| creator | Khoyratty, Shahnoo Dupont, Joëlle Lacoste, Sandrine Palama, Tony Lionel Choi, Young Hae Kim, Hye Kyong Payet, Bertrand Grisoni, Michel Fouillaud, Mireille Verpoorte, Robert Kodja, Hippolyte |
| description | The objective of the work was to characterize fungal endophytes from aerial parts of Vanilla planifolia. Also, to establish their biotransformation abilities of flavor-related metabolites. This was done in order to find a potential role of endophytes on vanilla flavors.
Twenty three MOTUs were obtained, representing 6 fungal classes. Fungi from green pods were cultured on mature green pod based media for 30 days followed by (1)H NMR and HPLC-DAD analysis. All fungi from pods consumed metabolized vanilla flavor phenolics. Though Fusarium proliferatum was recovered more often (37.6% of the isolates), it is Pestalotiopsis microspora (3.0%) that increased the absolute amounts (quantified by (1)H NMR in μmol/g DW green pods) of vanillin (37.0 × 10(-3)), vanillyl alcohol (100.0 × 10(-3)), vanillic acid (9.2 × 10(-3)) and p-hydroxybenzoic acid (87.9 × 10(-3)) by significant amounts.
All plants studied contained endophytic fungi and the isolation of the endophytes was conducted from plant organs at nine sites in Réunion Island including under shade house and undergrowth conditions. Endophytic variation occured between cultivation practices and the type of organ. Given the physical proximity of fungi inside pods, endophytic biotransformation may contribute to the complexity of vanilla flavors. |
| doi_str_mv | 10.1186/s12870-015-0522-5 |
| format | Article |
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Twenty three MOTUs were obtained, representing 6 fungal classes. Fungi from green pods were cultured on mature green pod based media for 30 days followed by (1)H NMR and HPLC-DAD analysis. All fungi from pods consumed metabolized vanilla flavor phenolics. Though Fusarium proliferatum was recovered more often (37.6% of the isolates), it is Pestalotiopsis microspora (3.0%) that increased the absolute amounts (quantified by (1)H NMR in μmol/g DW green pods) of vanillin (37.0 × 10(-3)), vanillyl alcohol (100.0 × 10(-3)), vanillic acid (9.2 × 10(-3)) and p-hydroxybenzoic acid (87.9 × 10(-3)) by significant amounts.
All plants studied contained endophytic fungi and the isolation of the endophytes was conducted from plant organs at nine sites in Réunion Island including under shade house and undergrowth conditions. Endophytic variation occured between cultivation practices and the type of organ. Given the physical proximity of fungi inside pods, endophytic biotransformation may contribute to the complexity of vanilla flavors.</description><identifier>ISSN: 1471-2229</identifier><identifier>EISSN: 1471-2229</identifier><identifier>DOI: 10.1186/s12870-015-0522-5</identifier><identifier>PMID: 26070309</identifier><language>eng</language><publisher>England: BioMed Central</publisher><subject>Biodiversity ; Biotransformation - drug effects ; Chromatography, High Pressure Liquid ; Cluster Analysis ; Culture Media - pharmacology ; Endophytes - isolation & purification ; Fungi - isolation & purification ; Geography ; Life Sciences ; Metabolome - drug effects ; Molecular Sequence Data ; Odorants ; Organ Specificity - drug effects ; Phylogeny ; Principal Component Analysis ; Proton Magnetic Resonance Spectroscopy ; Reunion ; Vanilla - drug effects ; Vanilla - microbiology ; Vegetal Biology</subject><ispartof>BMC plant biology, 2015-06, Vol.15 (1), p.142-142, Article 142</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Khoyratty et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-42c3465130dd0bebdb2f63031ea45c62f9d80c0be1d8d6b41724fdb262ed5e933</citedby><cites>FETCH-LOGICAL-c433t-42c3465130dd0bebdb2f63031ea45c62f9d80c0be1d8d6b41724fdb262ed5e933</cites><orcidid>0000-0002-4519-4502 ; 0000-0001-9867-9098 ; 0000-0002-0562-2154</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4465486/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4465486/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26070309$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01397514$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Khoyratty, Shahnoo</creatorcontrib><creatorcontrib>Dupont, Joëlle</creatorcontrib><creatorcontrib>Lacoste, Sandrine</creatorcontrib><creatorcontrib>Palama, Tony Lionel</creatorcontrib><creatorcontrib>Choi, Young Hae</creatorcontrib><creatorcontrib>Kim, Hye Kyong</creatorcontrib><creatorcontrib>Payet, Bertrand</creatorcontrib><creatorcontrib>Grisoni, Michel</creatorcontrib><creatorcontrib>Fouillaud, Mireille</creatorcontrib><creatorcontrib>Verpoorte, Robert</creatorcontrib><creatorcontrib>Kodja, Hippolyte</creatorcontrib><title>Fungal endophytes of Vanilla planifolia across Réunion Island: isolation, distribution and biotransformation</title><title>BMC plant biology</title><addtitle>BMC Plant Biol</addtitle><description>The objective of the work was to characterize fungal endophytes from aerial parts of Vanilla planifolia. Also, to establish their biotransformation abilities of flavor-related metabolites. This was done in order to find a potential role of endophytes on vanilla flavors.
Twenty three MOTUs were obtained, representing 6 fungal classes. Fungi from green pods were cultured on mature green pod based media for 30 days followed by (1)H NMR and HPLC-DAD analysis. All fungi from pods consumed metabolized vanilla flavor phenolics. Though Fusarium proliferatum was recovered more often (37.6% of the isolates), it is Pestalotiopsis microspora (3.0%) that increased the absolute amounts (quantified by (1)H NMR in μmol/g DW green pods) of vanillin (37.0 × 10(-3)), vanillyl alcohol (100.0 × 10(-3)), vanillic acid (9.2 × 10(-3)) and p-hydroxybenzoic acid (87.9 × 10(-3)) by significant amounts.
All plants studied contained endophytic fungi and the isolation of the endophytes was conducted from plant organs at nine sites in Réunion Island including under shade house and undergrowth conditions. Endophytic variation occured between cultivation practices and the type of organ. Given the physical proximity of fungi inside pods, endophytic biotransformation may contribute to the complexity of vanilla flavors.</description><subject>Biodiversity</subject><subject>Biotransformation - drug effects</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cluster Analysis</subject><subject>Culture Media - pharmacology</subject><subject>Endophytes - isolation & purification</subject><subject>Fungi - isolation & purification</subject><subject>Geography</subject><subject>Life Sciences</subject><subject>Metabolome - drug effects</subject><subject>Molecular Sequence Data</subject><subject>Odorants</subject><subject>Organ Specificity - drug effects</subject><subject>Phylogeny</subject><subject>Principal Component Analysis</subject><subject>Proton Magnetic Resonance Spectroscopy</subject><subject>Reunion</subject><subject>Vanilla - drug effects</subject><subject>Vanilla - microbiology</subject><subject>Vegetal Biology</subject><issn>1471-2229</issn><issn>1471-2229</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUV1L5TAQDeLix9Uf4IvkUcHuZvLV1gdBRFfhwoKoryFtUm-kTa5Ne8Gf5O_wj23qdUX3JZmZc-ZMMgehAyA_AQr5KwItcpIREBkRlGZiA-0AzyGjlJabX-JttBvjEyGQF7zcQttUkpwwUu6g7mr0j7rF1puwXLwMNuLQ4AftXdtqvGxT0ITWaazrPsSIb99eR--CxzcxYeYUuxhaPaTKCTYuDr2rxinDCcSVC0OvfWxC371z9tCPRrfR7n_cM3R_dXl3cZ3N__y-uTifZzVnbMg4rRmXAhgxhlS2MhVtJCMMrOailrQpTUHqhIApjKw45JQ3iSSpNcKWjM3Q2Vp3OVadNbX16R2tWvau0_2LCtqp74h3C_UYVoqnsbyQSeB4LbD4r-36fK6mGgFW5gL4ChL36GNYH55HGwfVuVjbtD9vwxgVyKJkQKdzhmBNfV9mb5tPbSBqslStLU3yQk2WKpF6Dr_-5bPjn4fsL-L1n9A</recordid><startdate>20150614</startdate><enddate>20150614</enddate><creator>Khoyratty, Shahnoo</creator><creator>Dupont, Joëlle</creator><creator>Lacoste, Sandrine</creator><creator>Palama, Tony Lionel</creator><creator>Choi, Young Hae</creator><creator>Kim, Hye Kyong</creator><creator>Payet, Bertrand</creator><creator>Grisoni, Michel</creator><creator>Fouillaud, Mireille</creator><creator>Verpoorte, Robert</creator><creator>Kodja, Hippolyte</creator><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4519-4502</orcidid><orcidid>https://orcid.org/0000-0001-9867-9098</orcidid><orcidid>https://orcid.org/0000-0002-0562-2154</orcidid></search><sort><creationdate>20150614</creationdate><title>Fungal endophytes of Vanilla planifolia across Réunion Island: isolation, distribution and biotransformation</title><author>Khoyratty, Shahnoo ; Dupont, Joëlle ; Lacoste, Sandrine ; Palama, Tony Lionel ; Choi, Young Hae ; Kim, Hye Kyong ; Payet, Bertrand ; Grisoni, Michel ; Fouillaud, Mireille ; Verpoorte, Robert ; Kodja, Hippolyte</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-42c3465130dd0bebdb2f63031ea45c62f9d80c0be1d8d6b41724fdb262ed5e933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biodiversity</topic><topic>Biotransformation - drug effects</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cluster Analysis</topic><topic>Culture Media - pharmacology</topic><topic>Endophytes - isolation & purification</topic><topic>Fungi - isolation & purification</topic><topic>Geography</topic><topic>Life Sciences</topic><topic>Metabolome - drug effects</topic><topic>Molecular Sequence Data</topic><topic>Odorants</topic><topic>Organ Specificity - drug effects</topic><topic>Phylogeny</topic><topic>Principal Component Analysis</topic><topic>Proton Magnetic Resonance Spectroscopy</topic><topic>Reunion</topic><topic>Vanilla - drug effects</topic><topic>Vanilla - microbiology</topic><topic>Vegetal Biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khoyratty, Shahnoo</creatorcontrib><creatorcontrib>Dupont, Joëlle</creatorcontrib><creatorcontrib>Lacoste, Sandrine</creatorcontrib><creatorcontrib>Palama, Tony Lionel</creatorcontrib><creatorcontrib>Choi, Young Hae</creatorcontrib><creatorcontrib>Kim, Hye Kyong</creatorcontrib><creatorcontrib>Payet, Bertrand</creatorcontrib><creatorcontrib>Grisoni, Michel</creatorcontrib><creatorcontrib>Fouillaud, Mireille</creatorcontrib><creatorcontrib>Verpoorte, Robert</creatorcontrib><creatorcontrib>Kodja, Hippolyte</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC plant biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khoyratty, Shahnoo</au><au>Dupont, Joëlle</au><au>Lacoste, Sandrine</au><au>Palama, Tony Lionel</au><au>Choi, Young Hae</au><au>Kim, Hye Kyong</au><au>Payet, Bertrand</au><au>Grisoni, Michel</au><au>Fouillaud, Mireille</au><au>Verpoorte, Robert</au><au>Kodja, Hippolyte</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fungal endophytes of Vanilla planifolia across Réunion Island: isolation, distribution and biotransformation</atitle><jtitle>BMC plant biology</jtitle><addtitle>BMC Plant Biol</addtitle><date>2015-06-14</date><risdate>2015</risdate><volume>15</volume><issue>1</issue><spage>142</spage><epage>142</epage><pages>142-142</pages><artnum>142</artnum><issn>1471-2229</issn><eissn>1471-2229</eissn><abstract>The objective of the work was to characterize fungal endophytes from aerial parts of Vanilla planifolia. Also, to establish their biotransformation abilities of flavor-related metabolites. This was done in order to find a potential role of endophytes on vanilla flavors.
Twenty three MOTUs were obtained, representing 6 fungal classes. Fungi from green pods were cultured on mature green pod based media for 30 days followed by (1)H NMR and HPLC-DAD analysis. All fungi from pods consumed metabolized vanilla flavor phenolics. Though Fusarium proliferatum was recovered more often (37.6% of the isolates), it is Pestalotiopsis microspora (3.0%) that increased the absolute amounts (quantified by (1)H NMR in μmol/g DW green pods) of vanillin (37.0 × 10(-3)), vanillyl alcohol (100.0 × 10(-3)), vanillic acid (9.2 × 10(-3)) and p-hydroxybenzoic acid (87.9 × 10(-3)) by significant amounts.
All plants studied contained endophytic fungi and the isolation of the endophytes was conducted from plant organs at nine sites in Réunion Island including under shade house and undergrowth conditions. Endophytic variation occured between cultivation practices and the type of organ. Given the physical proximity of fungi inside pods, endophytic biotransformation may contribute to the complexity of vanilla flavors.</abstract><cop>England</cop><pub>BioMed Central</pub><pmid>26070309</pmid><doi>10.1186/s12870-015-0522-5</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-4519-4502</orcidid><orcidid>https://orcid.org/0000-0001-9867-9098</orcidid><orcidid>https://orcid.org/0000-0002-0562-2154</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biodiversity Biotransformation - drug effects Chromatography, High Pressure Liquid Cluster Analysis Culture Media - pharmacology Endophytes - isolation & purification Fungi - isolation & purification Geography Life Sciences Metabolome - drug effects Molecular Sequence Data Odorants Organ Specificity - drug effects Phylogeny Principal Component Analysis Proton Magnetic Resonance Spectroscopy Reunion Vanilla - drug effects Vanilla - microbiology Vegetal Biology |
| title | Fungal endophytes of Vanilla planifolia across Réunion Island: isolation, distribution and biotransformation |
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