High‐frequency voltage oscillations in cultured astrocytes

Because of their close interaction with neuronal physiology, astrocytes can modulate brain function in multiple ways. Here, we demonstrate a yet unknown astrocytic phenomenon: Astrocytes cultured on microelectrode arrays (MEAs) exhibited extracellular voltage fluctuations in a broad frequency spectrum (100–600 Hz) after electrical stimulation. These aperiodic high‐frequency oscillations (HFOs) could last several seconds and did not spread across the MEA. The voltage‐gated calcium channel antagonist cilnidipine dose‐dependently decreased the power of the oscillations. While intracellular calcium was pivotal, incubation with bafilomycin A1 showed that vesicular release of transmitters played only a minor role in the emergence of HFOs. Gap junctions and volume‐regulated anionic channels had just as little functional impact, which was demonstrated by the addition of carbenoxolone (100 μmol/L) and NPPB (100 μmol/L). Hyperpolarization with low potassium in the extracellular solution (2 mmol/L) dramatically raised oscillation power. A similar effect was seen when we added extra sodium (+50 mmol/L) or if we replaced it with NMDG+ (50 mmol/L). The purinergic receptor antagonist PPADS suppressed the oscillation power, while the agonist ATP (100 μmol/L) had only an increasing effect when the bath solution pH was slightly lowered to pH 7.2. From these observations, we conclude that astrocytic voltage oscillations are triggered by activation of voltage‐gated calcium channels and driven by a downstream influx of cations through channels that are permeable for large ions such as NMDG+. Most likely candidates are subtypes of pore‐forming P2X channels with a low affinity for ATP. Astrocytes cultured on microelectrode arrays exhibited robust high‐frequency (100–600 Hz) extracellular voltage fluctuations after electrical stimulation. These voltage oscillations had amplitudes in the range of extracellularly recorded action potentials (~50 μV), were locally restricted to the site of the stimulus, lasted several seconds, and were highly susceptible to changes in the extracellular medium, e.g., [Ca2+]e and pH. The oscillations were triggered by activation of voltage‐gated Ca2+‐channels and driven by cation influx through channels with large membrane pores, presumably P2X channels.