Drawbacks in the use of unconventional hydrophobic anhydrides for histone derivatization in bottom-up proteomics PTM analysis

MS‐based proteomics has become the most utilized tool to characterize histone PTMs. Since histones are highly enriched in lysine and arginine residues, lysine derivatization has been developed to prevent the generation of short peptides (

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Bibliographische Detailangaben
Veröffentlicht in:	Proteomics (Weinheim) 2015-05, Vol.15 (9), p.1459-1469
Hauptverfasser:	Sidoli, Simone, Yuan, Zuo-Fei, Lin, Shu, Karch, Kelly, Wang, Xiaoshi, Bhanu, Natarajan, Arnaudo, Anna M., Britton, Laura-Mae, Cao, Xing-Jun, Gonzales-Cope, Michelle, Han, Yumiao, Liu, Shichong, Molden, Rosalynn C., Wein, Samuel, Afjehi-Sadat, Leila, Garcia, Benjamin A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Anhydride Anhydrides - chemistry Bottom-up proteomics Chromatography, High Pressure Liquid - methods HeLa Cells Histones Histones - chemistry Humans Hydrophobic and Hydrophilic Interactions Ionization Mass spectrometry Molecular Sequence Data Peptides Peptides - analysis Protein derivatization Proteomics Proteomics - methods Retention Retention time Tandem Mass Spectrometry - methods Technology
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Beschreibung
Zusammenfassung:	MS‐based proteomics has become the most utilized tool to characterize histone PTMs. Since histones are highly enriched in lysine and arginine residues, lysine derivatization has been developed to prevent the generation of short peptides (
ISSN:	1615-9853 1615-9861
DOI:	10.1002/pmic.201400483