Comparison of the Vidas C. difficile GDH Automated Enzyme-Linked Fluorescence Immunoassay (ELFA) with Another Commercial Enzyme Immunoassay (EIA) (Quik Chek-60), Two Selective Media, and a PCR Assay for gluD for Detection of Clostridium difficile in Fecal Samples
Prevention and management of Clostridium difficile infection (CDI) can be improved by rapid and reliable diagnostics. The Vidas C. difficile glutamate dehydrogenase assay had performance comparable to that of the Quik Chek-60 assay (overall agreement, 95%) and a sensitivity of >93%; thus, it is s...
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| Veröffentlicht in: | Journal of clinical microbiology 2015-06, Vol.53 (6), p.1931-1934 |
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| container_end_page | 1934 |
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| container_issue | 6 |
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| container_title | Journal of clinical microbiology |
| container_volume | 53 |
| creator | Davies, K A Berry, C E Morris, K A Smith, R Young, S Davis, T E Fuller, D D Buckner, R J Wilcox, M H |
| description | Prevention and management of Clostridium difficile infection (CDI) can be improved by rapid and reliable diagnostics. The Vidas C. difficile glutamate dehydrogenase assay had performance comparable to that of the Quik Chek-60 assay (overall agreement, 95%) and a sensitivity of >93%; thus, it is suitable as the first test in two-stage algorithms for a CDI diagnosis. |
| doi_str_mv | 10.1128/JCM.00649-15 |
| format | Article |
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The Vidas C. difficile glutamate dehydrogenase assay had performance comparable to that of the Quik Chek-60 assay (overall agreement, 95%) and a sensitivity of >93%; thus, it is suitable as the first test in two-stage algorithms for a CDI diagnosis.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.00649-15</identifier><identifier>PMID: 25788549</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Adolescent ; Adult ; ATP-Binding Cassette Transporters - genetics ; Bacterial Proteins - genetics ; Bacterial Typing Techniques - methods ; Bacteriology ; Child ; Child, Preschool ; Clostridium difficile ; Clostridium difficile - genetics ; Clostridium difficile - isolation & purification ; Culture Media ; Enterocolitis, Pseudomembranous - diagnosis ; Enterocolitis, Pseudomembranous - microbiology ; Enzyme-Linked Immunosorbent Assay - methods ; Feces - microbiology ; Humans ; Infant ; Infant, Newborn ; Male ; Middle Aged ; Polymerase Chain Reaction - methods ; Prospective Studies ; Reproducibility of Results ; Sensitivity and Specificity ; Young Adult</subject><ispartof>Journal of clinical microbiology, 2015-06, Vol.53 (6), p.1931-1934</ispartof><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</rights><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved. 2015 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-520480aa01b7d33b14523d25c19eff3e997d5ce332e032d943c8cd43cd5884bc3</citedby><cites>FETCH-LOGICAL-c417t-520480aa01b7d33b14523d25c19eff3e997d5ce332e032d943c8cd43cd5884bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432072/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432072/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25788549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Munson, E.</contributor><creatorcontrib>Davies, K A</creatorcontrib><creatorcontrib>Berry, C E</creatorcontrib><creatorcontrib>Morris, K A</creatorcontrib><creatorcontrib>Smith, R</creatorcontrib><creatorcontrib>Young, S</creatorcontrib><creatorcontrib>Davis, T E</creatorcontrib><creatorcontrib>Fuller, D D</creatorcontrib><creatorcontrib>Buckner, R J</creatorcontrib><creatorcontrib>Wilcox, M H</creatorcontrib><title>Comparison of the Vidas C. difficile GDH Automated Enzyme-Linked Fluorescence Immunoassay (ELFA) with Another Commercial Enzyme Immunoassay (EIA) (Quik Chek-60), Two Selective Media, and a PCR Assay for gluD for Detection of Clostridium difficile in Fecal Samples</title><title>Journal of clinical microbiology</title><addtitle>J Clin Microbiol</addtitle><description>Prevention and management of Clostridium difficile infection (CDI) can be improved by rapid and reliable diagnostics. The Vidas C. difficile glutamate dehydrogenase assay had performance comparable to that of the Quik Chek-60 assay (overall agreement, 95%) and a sensitivity of >93%; thus, it is suitable as the first test in two-stage algorithms for a CDI diagnosis.</description><subject>Adolescent</subject><subject>Adult</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Typing Techniques - methods</subject><subject>Bacteriology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Clostridium difficile</subject><subject>Clostridium difficile - genetics</subject><subject>Clostridium difficile - isolation & purification</subject><subject>Culture Media</subject><subject>Enterocolitis, Pseudomembranous - diagnosis</subject><subject>Enterocolitis, Pseudomembranous - microbiology</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Feces - microbiology</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Prospective Studies</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Young Adult</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUk1vEzEUXBCoDYUbZ-RjKmWDvbb341Ip2iRtUCo-WhC3lWO_bUy862Dvtiq_HjcJVeHCwfazPDN-8zRR9JbgMSFJ_v5DeTnGOGVFTPjzaEBwkcdpir-_iAYYFzwmhGbH0Svvf2BMGOP8KDpOeJbnnBWDZ0elbbbCaW9bZGvUrQF900p4VI6R0nWtpTaAzqcXaNJ3thEdKDRrf903EC91uwm3uemtAy-hlYAWTdO3Vngv7tFwtpxPTtGd7tZo0tog7VD4rQEntTAHlX8Yi0AYfu71BpVr2MQpPh2h6zuLrsCA7PQtoEtQWoyQaBUS6FP5BU121No6dGP66a6YQveA3lsqjfWd00r3zRNHukVzkKGNK9FsDfjX0ctaGA9vDudJ9HU-uy4v4uXH80U5WcaSkayLeYJZjoXAZJUpSleE8YSqhEtSQF1TKIpMcQmUJoBpogpGZS5V2BXPc7aS9CQ62-tu-1UDKkytc8JUW6cb4e4rK3T190ur19WNva0YownOkiAwPAg4-7MH31WNDsM3RrRge1-RDBdhMZb9H5rmJEkJJThAR3uodNZ7B_VjRwRXDzGrQsyqXcwqwgP83VMXj-A_uaK_AfUdzks</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Davies, K A</creator><creator>Berry, C E</creator><creator>Morris, K A</creator><creator>Smith, R</creator><creator>Young, S</creator><creator>Davis, T E</creator><creator>Fuller, D D</creator><creator>Buckner, R J</creator><creator>Wilcox, M H</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>5PM</scope></search><sort><creationdate>20150601</creationdate><title>Comparison of the Vidas C. difficile GDH Automated Enzyme-Linked Fluorescence Immunoassay (ELFA) with Another Commercial Enzyme Immunoassay (EIA) (Quik Chek-60), Two Selective Media, and a PCR Assay for gluD for Detection of Clostridium difficile in Fecal Samples</title><author>Davies, K A ; 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| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adolescent Adult ATP-Binding Cassette Transporters - genetics Bacterial Proteins - genetics Bacterial Typing Techniques - methods Bacteriology Child Child, Preschool Clostridium difficile Clostridium difficile - genetics Clostridium difficile - isolation & purification Culture Media Enterocolitis, Pseudomembranous - diagnosis Enterocolitis, Pseudomembranous - microbiology Enzyme-Linked Immunosorbent Assay - methods Feces - microbiology Humans Infant Infant, Newborn Male Middle Aged Polymerase Chain Reaction - methods Prospective Studies Reproducibility of Results Sensitivity and Specificity Young Adult |
| title | Comparison of the Vidas C. difficile GDH Automated Enzyme-Linked Fluorescence Immunoassay (ELFA) with Another Commercial Enzyme Immunoassay (EIA) (Quik Chek-60), Two Selective Media, and a PCR Assay for gluD for Detection of Clostridium difficile in Fecal Samples |
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