Near-infrared fluorescent proteins

Fluorescent proteins with emission wavelengths in the near-infrared and infrared range are in high demand for whole-body imaging techniques. Here we report near-infrared dimeric fluorescent proteins eqFP650 and eqFP670. To our knowledge, eqFP650 is the brightest fluorescent protein with emission max...

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Veröffentlicht in:	Nature methods 2010-10, Vol.7 (10), p.827-829
Hauptverfasser:	Chudakov, Dmitriy M, Shcherbo, Dmitry, Shemiakina, Irina I, Ryabova, Anastasiya V, Luker, Kathryn E, Schmidt, Bradley T, Souslova, Ekaterina A, Gorodnicheva, Tatiana V, Strukova, Lydia, Shidlovskiy, Konstantin M, Britanova, Olga V, Zaraisky, Andrey G, Lukyanov, Konstantin A, Loschenov, Victor B, Luker, Gary D
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1888/2249 631/1647/245/2225 Amino Acid Sequence Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biotechnology - instrumentation Biotechnology - methods brief-communication Embryo, Nonmammalian - cytology Embryo, Nonmammalian - drug effects Embryo, Nonmammalian - metabolism Emissions Escherichia coli - genetics Escherichia coli - growth & development Fluorescence Fluorescent proteins HeLa Cells Humans Infrared imaging systems Infrared Rays Life Sciences Luminescent Proteins - genetics Luminescent Proteins - toxicity Measurement Medical imaging Methods Mice Molecular Sequence Data Near infrared spectroscopy Properties Protein Multimerization Protein Stability Proteins Proteomics Research methodology Sequence Alignment Transfection Wavelength Wavelengths Whole Body Imaging - methods Xenopus laevis - genetics Xenopus laevis - metabolism Zebrafish - genetics Zebrafish - metabolism
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Beschreibung
Zusammenfassung:	Fluorescent proteins with emission wavelengths in the near-infrared and infrared range are in high demand for whole-body imaging techniques. Here we report near-infrared dimeric fluorescent proteins eqFP650 and eqFP670. To our knowledge, eqFP650 is the brightest fluorescent protein with emission maximum above 635 nm, and eqFP670 displays the most red-shifted emission maximum and high photostability.
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth.1501