Independent and coordinate trafficking of single Drosophila germ plasm mRNAs
Messenger RNA localization is a conserved mechanism for spatial control of protein synthesis, with key roles in generating cellular and developmental asymmetry. Whereas different transcripts may be targeted to the same subcellular domain, the extent to which their localization is coordinated is uncl...
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Veröffentlicht in: | Nature cell biology 2015-05, Vol.17 (5), p.558-568 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Messenger RNA localization is a conserved mechanism for spatial control of protein synthesis, with key roles in generating cellular and developmental asymmetry. Whereas different transcripts may be targeted to the same subcellular domain, the extent to which their localization is coordinated is unclear. Using quantitative single-molecule imaging, we analysed the assembly of
Drosophila
germ plasm mRNA granules inherited by nascent germ cells. We find that the germ-cell-destined transcripts
nanos
,
cyclin B
and
polar granule component
travel within the oocyte as ribonucleoprotein particles containing single mRNA molecules but co-assemble into multi-copy heterogeneous granules selectively at the posterior of the oocyte. The stoichiometry and dynamics of assembly indicate a defined stepwise sequence. Our data suggest that co-packaging of these transcripts ensures their effective segregation to germ cells. In contrast, compartmentalization of the germline determinant
oskar
mRNA into different granules limits its entry into germ cells. This exclusion is required for proper germline development.
Gavis and colleagues characterize RNP trafficking and assembly using quantitative single-molecule imaging and find mRNAs destined for the germ cells are assembled differently from oskar, the germline determinant mRNA. |
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ISSN: | 1465-7392 1476-4679 |
DOI: | 10.1038/ncb3143 |