Establishment of a counter-selectable markerless mutagenesis system in Veillonella atypica

Establishment of a counter-selectable markerless mutagenesis system in Veillonella atypica

Using an alternative sigma factor ecf3 as target, we successfully established the first markerless mutagenesis system in the Veillonella genus. This system will be a valuable tool for mutagenesis of multiple genes for gene function analysis as well as for gene regulation studies in Veillonella. •The...

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Veröffentlicht in:Journal of microbiological methods 2015-05, Vol.112, p.70-72
Hauptverfasser: Zhou, Peng, Li, Xiaoli, Qi, Fengxia
Format: Artikel
Sprache:eng
Schlagworte:
genes
Genetics, Microbial - methods
Markerless
Molecular Biology - methods
Mutagenesis
PheS
Selection, Genetic
Sigma Factor - genetics
sigma factors
Veillonella
Veillonella - genetics
Veillonella atypica
Veillonellae
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Zusammenfassung:Using an alternative sigma factor ecf3 as target, we successfully established the first markerless mutagenesis system in the Veillonella genus. This system will be a valuable tool for mutagenesis of multiple genes for gene function analysis as well as for gene regulation studies in Veillonella. •The first markerless system in Veillonella•A versatile mutagenesis system•Counter-selection
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2015.03.010