A pilot evaluation of external quality assessment of GenoType MTBDRplus versions 1 and 2 using dried culture spot material
Dried culture spots (DCS) of inactivated Mycobacteria strains designed as part of an external quality assessment (EQA) program for the GeneXpert system has applications to other molecular tuberculosis (TB) diagnostic platforms. DCS tested on the GenoType MTBDRplus and Mycobacterium CM assays perform...
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Veröffentlicht in: | Journal of clinical microbiology 2015-04, Vol.53 (4), p.1365-1367 |
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creator | Gous, N Isherwood, L E David, A Stevens, W Scott, L E |
description | Dried culture spots (DCS) of inactivated Mycobacteria strains designed as part of an external quality assessment (EQA) program for the GeneXpert system has applications to other molecular tuberculosis (TB) diagnostic platforms. DCS tested on the GenoType MTBDRplus and Mycobacterium CM assays performed well with MTBDRplus version 2 but require increased bacterial concentration for use with version 1. |
doi_str_mv | 10.1128/JCM.03340-14 |
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DCS tested on the GenoType MTBDRplus and Mycobacterium CM assays performed well with MTBDRplus version 2 but require increased bacterial concentration for use with version 1.</description><subject>Bacteriological Techniques - methods</subject><subject>Bacteriological Techniques - standards</subject><subject>Genotype</subject><subject>Humans</subject><subject>Molecular Diagnostic Techniques</subject><subject>Mycobacteriology and Aerobic Actinomycetes</subject><subject>Mycobacterium - genetics</subject><subject>Mycobacterium - isolation & purification</subject><subject>Pilot Projects</subject><subject>Sensitivity and Specificity</subject><subject>Species Specificity</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE1P3DAQhq2qqCzQW8-Vj70E7PFH1hck2LZQBEJCW6m3aJzMUlfOB3G8YvvrSVVawWkO78zzaF7GPkhxLCUsT65WN8dCKS0Kqd-whRRuWVgrfrxlCyGcKaRU5T47SOmXEFJrY96xfTBWuFLBgv0-40OI_cRpizHjFPqO9xtOjxONHUb-kDGGaccxJUqppW76E19Q1693A_Gb9fnnuyHmxLc0pvk4ccmxazjwnEJ3z5sxUMPrHKc8Ek_DbGpxZgeMR2xvgzHR--d5yL5__bJeXRbXtxffVmfXxQDWTkUpLDpDwmlXS28bL4CEtEpaNMZrV0JN0GBTO-stAHhXL3Wp_caDg9qjOmSnf7lD9i019fzDiLEaxtDiuKt6DNXrpAs_q_t-W2llDVgxAz49A8b-IVOaqjakmmLEjvqcKmmtkQBLBfPqx5eu_5J_hasnq-uEgQ</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Gous, N</creator><creator>Isherwood, L E</creator><creator>David, A</creator><creator>Stevens, W</creator><creator>Scott, L E</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150401</creationdate><title>A pilot evaluation of external quality assessment of GenoType MTBDRplus versions 1 and 2 using dried culture spot material</title><author>Gous, N ; Isherwood, L E ; David, A ; Stevens, W ; Scott, L E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-706a95e0949c1b6db02e016316a55b4972ce2dadc96b6222b9c8474bfb292cba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bacteriological Techniques - methods</topic><topic>Bacteriological Techniques - standards</topic><topic>Genotype</topic><topic>Humans</topic><topic>Molecular Diagnostic Techniques</topic><topic>Mycobacteriology and Aerobic Actinomycetes</topic><topic>Mycobacterium - genetics</topic><topic>Mycobacterium - isolation & purification</topic><topic>Pilot Projects</topic><topic>Sensitivity and Specificity</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gous, N</creatorcontrib><creatorcontrib>Isherwood, L E</creatorcontrib><creatorcontrib>David, A</creatorcontrib><creatorcontrib>Stevens, W</creatorcontrib><creatorcontrib>Scott, L E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gous, N</au><au>Isherwood, L E</au><au>David, A</au><au>Stevens, W</au><au>Scott, L E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A pilot evaluation of external quality assessment of GenoType MTBDRplus versions 1 and 2 using dried culture spot material</atitle><jtitle>Journal of clinical microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>53</volume><issue>4</issue><spage>1365</spage><epage>1367</epage><pages>1365-1367</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><abstract>Dried culture spots (DCS) of inactivated Mycobacteria strains designed as part of an external quality assessment (EQA) program for the GeneXpert system has applications to other molecular tuberculosis (TB) diagnostic platforms. 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source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
subjects | Bacteriological Techniques - methods Bacteriological Techniques - standards Genotype Humans Molecular Diagnostic Techniques Mycobacteriology and Aerobic Actinomycetes Mycobacterium - genetics Mycobacterium - isolation & purification Pilot Projects Sensitivity and Specificity Species Specificity |
title | A pilot evaluation of external quality assessment of GenoType MTBDRplus versions 1 and 2 using dried culture spot material |
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