A pilot evaluation of external quality assessment of GenoType MTBDRplus versions 1 and 2 using dried culture spot material

Dried culture spots (DCS) of inactivated Mycobacteria strains designed as part of an external quality assessment (EQA) program for the GeneXpert system has applications to other molecular tuberculosis (TB) diagnostic platforms. DCS tested on the GenoType MTBDRplus and Mycobacterium CM assays perform...

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Veröffentlicht in:	Journal of clinical microbiology 2015-04, Vol.53 (4), p.1365-1367
Hauptverfasser:	Gous, N, Isherwood, L E, David, A, Stevens, W, Scott, L E
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological Techniques - methods Bacteriological Techniques - standards Genotype Humans Molecular Diagnostic Techniques Mycobacteriology and Aerobic Actinomycetes Mycobacterium - genetics Mycobacterium - isolation & purification Pilot Projects Sensitivity and Specificity Species Specificity
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Beschreibung
Zusammenfassung:	Dried culture spots (DCS) of inactivated Mycobacteria strains designed as part of an external quality assessment (EQA) program for the GeneXpert system has applications to other molecular tuberculosis (TB) diagnostic platforms. DCS tested on the GenoType MTBDRplus and Mycobacterium CM assays performed well with MTBDRplus version 2 but require increased bacterial concentration for use with version 1.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.03340-14