Variola virus-specific diagnostic assays: characterization, sensitivity, and specificity

A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must con...

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Veröffentlicht in:	Journal of clinical microbiology 2015-04, Vol.53 (4), p.1406-1410
Hauptverfasser:	Kondas, Ashley V, Olson, Victoria A, Li, Yu, Abel, Jason, Laker, Miriam, Rose, Laura, Wilkins, Kimberly, Turner, Jonathan, Kline, Richard, Damon, Inger K
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence DNA, Viral - genetics Molecular Sequence Data Real-Time Polymerase Chain Reaction - methods Sensitivity and Specificity Species Specificity Variola Variola virus - isolation & purification Virology
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Beschreibung
Zusammenfassung:	A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reassessed as other closely related viruses are identified.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.03613-14