DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor

To determine the DNA binding activity and protein levels of the Ku70/80 heterodimer, the functional mediator of the NHEJ activity, in human colorectal carcinogenesis. The Ku70/80 DNA-binding activity was determined by electrophoretic mobility shift assays in 20 colon adenoma and 15 colorectal cancer samples as well as matched normal colonic tissues. Nuclear and cytoplasmic protein expression was determined by immunohistochemistry and Western blot analysis. A statistically significant difference was found in both adenomas and carcinomas as compared to matched normal colonic mucosa (P