Effect of Growth Conditions on DNA-Membrane Attachment in Escherichia coli

DNA-membrane attachment in cultures of E. coli B/r is found to depend on the pattern of DNA synthesis. (a) When the number of replication points increases, the fraction of the DNA that is membrane bound also increases. The relationship between the amount of DNA-membrane attachment and the number of...

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Veröffentlicht in:	Proc. Natl. Acad. Sci. U.S.A., v. 72, no. 6, pp. 2446-2450 v. 72, no. 6, pp. 2446-2450, 1975-06, Vol.72 (6), p.2446-2450
Hauptverfasser:	Parker, Dorothy Lundquist, Glaser, Donald A.
Format:	Artikel
Sprache:	eng
Schlagworte:	560131 -Biomedical Sciences, Applied Studies-Radiation Effects-Radiation Effects on Microorganisms-Basic Studies Bacteria BIOLOGICAL REPAIR Cell growth Cell Membrane - metabolism Cell Membrane - radiation effects CELL MEMBRANES CHROMOSOMES DNA Repair DNA Replication DNA, Bacterial - biosynthesis DNA, Bacterial - metabolism DNA- BIOSYNTHESIS Doubling time Escherichia coli Escherichia coli - growth & development Escherichia coli - metabolism Escherichia coli - radiation effects Escherichia coli - ultrastructure ESCHERICHIA COLI- BIOLOGICAL RADIATION EFFECTS Irradiation Mathematics Modeling N48310 -Life Sciences-Radiation Effects on Microorganisms- -Basic Studies P branes Salts Thymine - metabolism ULTRAVIOLET RADIATION Ultraviolet Rays
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Zusammenfassung:	DNA-membrane attachment in cultures of E. coli B/r is found to depend on the pattern of DNA synthesis. (a) When the number of replication points increases, the fraction of the DNA that is membrane bound also increases. The relationship between the amount of DNA-membrane attachment and the number of replication points is most consistent with attachment of all replication points and attachment of at least one other chromosomal site; it is inconsistent with attachment of the replication points only, the chromosomal origin only, or the chromosomal terminus only. (b) When repair DNA synthesis is stimulated by irradiation with ultraviolet light, the DNA-membrane attachment remains unchanged.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.72.6.2446