Pulsed Laser Activated Cell Sorting with Three Dimensional Sheathless Inertial Focusing

Pulsed Laser Activated Cell Sorting with Three Dimensional Sheathless Inertial Focusing

A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells with high purity at high‐throughput using a single layer PDMS channel that can be easily fabricated at low cost. Sort purity of >90% is achieved at a throughput of 10 000 particles s−1 without...

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Veröffentlicht in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2014-05, Vol.10 (9), p.1746-1751
Hauptverfasser: Chen, Yue, Chung, Aram J., Wu, Ting-Hsiang, Teitell, Michael A., Di Carlo, Dino, Chiou, Pei-Yu
Format: Artikel
Sprache:eng
Schlagworte:
Activated
Cell Line, Tumor
Cell Separation - methods
cell sorting
Channels
Humans
Inertial
inertial focusing
lab-on-a-chip devices
Lasers
Low cost
Microfluidic Analytical Techniques - methods
microfluidics
Nanotechnology
Pulsed lasers
Purity
Silicone resins
Three dimensional
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Beschreibung
Zusammenfassung:A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells with high purity at high‐throughput using a single layer PDMS channel that can be easily fabricated at low cost. Sort purity of >90% is achieved at a throughput of 10 000 particles s−1 without severe dilution effects from high volume sheath flows.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201302885