A Barcode-Free Combinatorial Screening Platform for Matrix Metalloproteinase Screening

Application of droplet microfluidics to combinatorial screening applications remains elusive because of the need for composition-identifying unique barcodes. Here we propose a barcode-free continuous flow droplet microfluidic platform to suit the requirements of combinatorial screening applications....

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Veröffentlicht in:	Analytical chemistry (Washington) 2015-02, Vol.87 (3), p.1950-1956
Hauptverfasser:	Rane, Tushar D, Zec, Helena C, Wang, Tza-Huei
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Bar codes Combinatorial analysis Continuous flow Droplets Enzyme Assays - instrumentation Enzymes Equipment Design Matrix metalloproteinases Matrix Metalloproteinases - analysis Matrix Metalloproteinases - metabolism Medical screening Microfluidic Analytical Techniques - instrumentation Microfluidics Platforms Screening
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Zusammenfassung:	Application of droplet microfluidics to combinatorial screening applications remains elusive because of the need for composition-identifying unique barcodes. Here we propose a barcode-free continuous flow droplet microfluidic platform to suit the requirements of combinatorial screening applications. We demonstrate robust and repeatable functioning of this platform with matrix metalloproteinase activity screening as a sample application.
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac504330x