Isolation and biological and molecular characterization of Toxoplasma gondii from canine cutaneous toxoplasmosis in Brazil

Cutaneous toxoplasmosis is a rare manifestation. This study represents a case report of an immunosuppressed dog that developed nodular dermal lesions caused by Toxoplasma gondii. The isolate (TgDgBr20) was characterized as mouse virulent and was genotyped as type BrI (ToxoDB genotype 6) using PCR-re...

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Veröffentlicht in:	Journal of clinical microbiology 2014-12, Vol.52 (12), p.4419-4420
Hauptverfasser:	Pena, Hilda F J, Moroz, Ludmila R, Sozigan, Rita K B, Ajzenberg, Daniel, Carvalho, Fernando R, Mota, Caroline M, Mineo, Tiago W P, Marcili, Arlei
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brazil Case Reports Disease Models, Animal DNA, Protozoan - genetics Dog Diseases - parasitology Dogs Genotype Immunocompromised Host Life Sciences Male Mice Microsatellite Repeats Polymorphism, Restriction Fragment Length Santé publique et épidémiologie Toxoplasma - classification Toxoplasma - genetics Toxoplasma - isolation & purification Toxoplasma - physiology Toxoplasma gondii Toxoplasmosis, Animal - parasitology Virulence
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Beschreibung
Zusammenfassung:	Cutaneous toxoplasmosis is a rare manifestation. This study represents a case report of an immunosuppressed dog that developed nodular dermal lesions caused by Toxoplasma gondii. The isolate (TgDgBr20) was characterized as mouse virulent and was genotyped as type BrI (ToxoDB genotype 6) using PCR-restriction fragment length polymorphism (RFLP) and as Africa 1 through microsatellite analysis.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.02001-14