Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development

Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development

In order to ascertain optimum conditions for biocatalytic processes carried out in vitro , we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP conce...

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Veröffentlicht in:Scientific reports 2015-01, Vol.5 (1), p.8135-8135, Article 8135
Hauptverfasser: Hu, Jie-Bi, Chen, Ting-Ru, Chen, Yu-Chie, Urban, Pawel L.
Format: Artikel
Sprache:eng
Schlagworte:
631/45/603
639/638/11/872
9/10
Adenosine triphosphate
Adenosine Triphosphate - metabolism
Algorithms
Apyrase
Apyrase - metabolism
ATP
Compensation
Control algorithms
Electronics - instrumentation
Enzymes
Experiments
Humanities and Social Sciences
Microtechnology - instrumentation
Microtechnology - methods
multidisciplinary
Optics and Photonics - instrumentation
Science
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Beschreibung
Zusammenfassung:In order to ascertain optimum conditions for biocatalytic processes carried out in vitro , we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2–48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep08135