Characterization of cross-reactive norovirus-specific monoclonal antibodies

Noroviruses (NoVs) commonly cause acute gastroenteritis outbreaks. Broadly reactive diagnostic assays are essential for rapid detection of NoV infections. We previously generated a panel of broadly reactive monoclonal antibodies (MAbs). We characterized MAb reactivities by use of virus-like particle...

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Veröffentlicht in:Clinical and vaccine immunology 2015-02, Vol.22 (2), p.160-167
Hauptverfasser: Kou, Baijun, Crawford, Sue E, Ajami, Nadim J, Czakó, Rita, Neill, Frederick H, Tanaka, Tomoyuki N, Kitamoto, Noritoshi, Palzkill, Timothy G, Estes, Mary K, Atmar, Robert L
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Sprache:eng
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Zusammenfassung:Noroviruses (NoVs) commonly cause acute gastroenteritis outbreaks. Broadly reactive diagnostic assays are essential for rapid detection of NoV infections. We previously generated a panel of broadly reactive monoclonal antibodies (MAbs). We characterized MAb reactivities by use of virus-like particles (VLPs) from 16 different NoV genotypes (6 from genogroup I [GI], 9 from GII, and 1 from GIV) coating a microtiter plate (direct enzyme-linked immunosorbent assay [ELISA]) and by Western blotting. MAbs were genotype specific or recognized multiple genotypes within a genogroup and between genogroups. We next applied surface plasmon resonance (SPR) analysis to measure MAb dissociation constants (Kd) as a surrogate for binding affinity; a Kd level of
ISSN:1556-6811
1556-679X
DOI:10.1128/cvi.00519-14