Comparison of test specificities of commercial antigen-based assays and in-house PCR methods for detection of rotavirus in stool specimens

Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, e...

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Veröffentlicht in:	Journal of clinical microbiology 2015-01, Vol.53 (1), p.295-297
Hauptverfasser:	Ye, S, Lambert, S B, Grimwood, K, Roczo-Farkas, S, Nimmo, G R, Sloots, T P, Kirkwood, C D, Whiley, D M
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Aged Aged, 80 and over Child Child, Preschool Feces - virology Female Humans Immunochromatography - methods Infant Infant, Newborn Male Middle Aged Polymerase Chain Reaction - methods Reproducibility of Results Rotavirus Rotavirus - genetics Rotavirus - isolation & purification Rotavirus Infections - diagnosis Rotavirus Infections - virology Sensitivity and Specificity Virology Young Adult
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Zusammenfassung:	Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.02251-14