DACT2 is frequently methylated in human gastric cancer and methylation of DACT2 activated Wnt signaling

DACT2 is frequently methylated in human gastric cancer and methylation of DACT2 activated Wnt signaling

Dapper, Dishevelled-associated antagonist of β-catenin (DACT), is a key regulator of Wnt signaling pathway. The purpose of this study is to explore the epigenetic changes and the function ofDACT2 in human gastric cancer (GC). Eight human gastric cancer cell lines, 167 cases of primary gastric cancer...

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Veröffentlicht in:American journal of cancer research 2014-01, Vol.4 (6), p.710-724
Hauptverfasser: Yu, Yuanzi, Yan, Wenji, Liu, Xuefeng, Jia, Yan, Cao, Baoping, Yu, Yingyan, Lv, Youyong, Brock, Malcolm V, Herman, Jame G, Licchesi, Julien, Yang, Yunsheng, Guo, Mingzhou
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Sprache:eng
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Zusammenfassung:Dapper, Dishevelled-associated antagonist of β-catenin (DACT), is a key regulator of Wnt signaling pathway. The purpose of this study is to explore the epigenetic changes and the function ofDACT2 in human gastric cancer (GC). Eight human gastric cancer cell lines, 167 cases of primary gastric cancer and 8 cases of normal gastric mucosa were involved in this study. In addition, methylation Specific PCR (MSP), semi-quantitative RT-PCR, colony formation assay, flow cytometry assay, siRNA, immunofluorescence techniques and xenograft mice models were employed. The results indicate that DACT2 is frequently methylated in human primary gastric cancer (55.7%), and that methylation of DACT2 is associated with lost or reduction in its expression (X(2) test, P
ISSN:2156-6976
2156-6976