High frame-rate multichannel beam-scanning microscopy based on Lissajous trajectories

A simple beam-scanning optical design based on Lissajous trajectory imaging is described for achieving up to kHz frame-rate optical imaging on multiple simultaneous data acquisition channels. In brief, two fast-scan resonant mirrors direct the optical beam on a circuitous trajectory through the fiel...

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Veröffentlicht in:Optics express 2014-10, Vol.22 (20), p.24224-24234
Hauptverfasser: Sullivan, Shane Z, Muir, Ryan D, Newman, Justin A, Carlsen, Mark S, Sreehari, Suhas, Doerge, Chris, Begue, Nathan J, Everly, R Michael, Bouman, Charles A, Simpson, Garth J
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Sprache:eng
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Zusammenfassung:A simple beam-scanning optical design based on Lissajous trajectory imaging is described for achieving up to kHz frame-rate optical imaging on multiple simultaneous data acquisition channels. In brief, two fast-scan resonant mirrors direct the optical beam on a circuitous trajectory through the field of view, with the trajectory repeat-time given by the least common multiplier of the mirror periods. Dicing the raw time-domain data into sub-trajectories combined with model-based image reconstruction (MBIR) 3D in-painting algorithms allows for effective frame-rates much higher than the repeat time of the Lissajous trajectory. Since sub-trajectory and full-trajectory imaging are simply different methods of analyzing the same data, both high-frame rate images with relatively low resolution and low frame rate images with high resolution are simultaneously acquired. The optical hardware required to perform Lissajous imaging represents only a minor modification to established beam-scanning hardware, combined with additional control and data acquisition electronics. Preliminary studies based on laser transmittance imaging and polarization-dependent second harmonic generation microscopy support the viability of the approach both for detection of subtle changes in large signals and for trace-light detection of transient fluctuations.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.22.024224