Circulating cell-free DNA in serum as a biomarker for diagnosis and prognostic prediction of colorectal cancer

Background: To verify whether the concentrations and integrity index of circulating cell-free DNA (ccf-DNA) in serum may be clinically useful for the diagnosis and progression monitoring of colorectal cancer (CRC) patients. Methods: Serum samples were collected from 104 with primary CRC, 85 with ope...

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Veröffentlicht in:	British journal of cancer 2014-10, Vol.111 (8), p.1482-1489
Hauptverfasser:	Hao, T B, Shi, W, Shen, X J, Qi, J, Wu, X H, Wu, Y, Tang, Y Y, Ju, S Q
Format:	Artikel
Sprache:	eng
Schlagworte:	692/53 692/699/67/1504/1885 692/700/139 692/700/1750 Antigens Base Sequence Biological and medical sciences Biomarkers Biomarkers, Tumor - blood Biomedical and Life Sciences Biomedicine Blood & organ donations Cancer Research Case-Control Studies Cell-Free System Clinical Study Colorectal cancer Colorectal Neoplasms - blood Colorectal Neoplasms - diagnosis Colorectal Neoplasms - pathology DNA - blood DNA Primers Drug Resistance Epidemiology Gastroenterology. Liver. Pancreas. Abdomen Hospitals Humans Medical prognosis Medical research Medical sciences Metastasis Molecular Medicine Multiple tumors. Solid tumors. Tumors in childhood (general aspects) Oncology Polyps Prognosis Real-Time Polymerase Chain Reaction Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Surgery Tumors
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Zusammenfassung:	Background: To verify whether the concentrations and integrity index of circulating cell-free DNA (ccf-DNA) in serum may be clinically useful for the diagnosis and progression monitoring of colorectal cancer (CRC) patients. Methods: Serum samples were collected from 104 with primary CRC, 85 with operated CRC, 16 with recurrent/metastatic CRC, 63 patients with intestinal polyps and 110 normal controls. Long (247 bp) and short (115 bp) DNA fragments in serum were detected by real-time quantitative PCR by amplifying the ALU repeats (ALU-qPCR). Serum carcinoembryonic antigen (CEA) level was detected by ARCHITECT assay. Results: The median absolute serum ALU115 and ALU247/115 in primary CRC group was significantly higher than those in intestinal polyp and normal control groups (both P
ISSN:	0007-0920 1532-1827
DOI:	10.1038/bjc.2014.470