Comparative analysis of optogenetic actuators in cultured astrocytes

Highlights • We compared six optogenetic tools to selectively stimulate and study astrocytes. • Channelrhodopsin-2 variants cause release of Ca2+ from intracellular stores. • Opto-GPCRs activate selective second messenger cascades, leading to [Ca2+ ] i rises. • Autocrine action of ATP mediates the b...

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Veröffentlicht in:	Cell calcium (Edinburgh) 2014-09, Vol.56 (3), p.208-214
Hauptverfasser:	Figueiredo, Melina, Lane, Samantha, Stout, Randy F, Liu, Beihui, Parpura, Vladimir, Teschemacher, Anja G, Kasparov, Sergey
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Advanced Basic Science Animals Astrocyte Astrocytes - cytology Astrocytes - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Calcium Calcium - metabolism Calcium Channels - physiology CatCh Cells, Cultured Channelrhodopsins ChR2 Luminescent Proteins - genetics Luminescent Proteins - metabolism Mutation - genetics OptoARs Optogenetics Rats Rats, Wistar Receptors, Adrenergic, alpha-1 - genetics Receptors, Adrenergic, alpha-1 - metabolism Receptors, Adrenergic, beta-2 - genetics Receptors, Adrenergic, beta-2 - metabolism Type C Phospholipases - metabolism
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Zusammenfassung:	Highlights • We compared six optogenetic tools to selectively stimulate and study astrocytes. • Channelrhodopsin-2 variants cause release of Ca2+ from intracellular stores. • Opto-GPCRs activate selective second messenger cascades, leading to [Ca2+ ] i rises. • Autocrine action of ATP mediates the bulk of [Ca2+ ] i signals evoked by opto-GPCRs. • Current optogenetic tools initiate relevant signalling events in astrocytes.
ISSN:	0143-4160 1532-1991
DOI:	10.1016/j.ceca.2014.07.007