Focused specificity of intestinal TH17 cells towards commensal bacterial antigens

Segmented filamentous bacteria drive the acquisition of the T H 17 phenotype in an antigen-specific manner; these findings begin to elucidate how gut-induced T H 17 cells can contribute to distal organ-specific autoimmune disease. T H 17 cell differentiation linked to intestinal bacteria Colonization of the small intestine by microbes such as segmented filamentous bacteria is known to enhance the induction of T-helper-17 (T H 17) cells, which are important factors in both mucosal defence and in autoimmune disease pathogenesis. Here Dan Littman and colleagues demonstrate that the vast majority of T H 17 cells in mice colonized with segmented filamentous bacteria are directed at antigens encoded by these bacteria, and identify specific bacterial epitopes that are recognized by T H 17 T-cell receptors. This work provides insights into how microbiota communicate with the host immune system, and suggests possible routes for developing novel mucosal vaccines. T-helper-17 (T H 17) cells have critical roles in mucosal defence and in autoimmune disease pathogenesis 1 , 2 , 3 . They are most abundant in the small intestine lamina propria, where their presence requires colonization of mice with microbiota 4 , 5 , 6 , 7 . Segmented filamentous bacteria (SFB) are sufficient to induce T H 17 cells and to promote T H 17-dependent autoimmune disease in animal models 8 , 9 , 10 , 11 , 12 , 13 , 14 . However, the specificity of T H 17 cells, the mechanism of their induction by distinct bacteria, and the means by which they foster tissue-specific inflammation remain unknown. Here we show that the T-cell antigen receptor (TCR) repertoire of intestinal T H 17 cells in SFB-colonized mice has minimal overlap with that of other intestinal CD4 + T cells and that most T H 17 cells, but not other T cells, recognize antigens encoded by SFB. T cells with antigen receptors specific for SFB-encoded peptides differentiated into RORγt-expressing T H 17 cells, even if SFB-colonized mice also harboured a strong T H 1 cell inducer, Listeria monocytogenes , in their intestine. The match of T-cell effector function with antigen specificity is thus determined by the type of bacteria that produce the antigen. These findings have significant implications for understanding how commensal microbiota contribute to organ-specific autoimmunity and for developing novel mucosal vaccines.