Genome-wide identification of CRISPR/Cas9 off-targets in human genome

Genome editing techniques have been rapidly developing in recent decades [1]. Among them, sitespecific cleavage of genomic loci in various organisms by homing endonucleases （HEases） [2], Zinc finger nucleases （ZFNs） [3], transcription activator-like effector nucleases （TALENs） [4], and most recently...

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Veröffentlicht in:	Cell research 2014-08, Vol.24 (8), p.1009-1012
Hauptverfasser:	Duan, Jinzhi, Lu, Guangqing, Xie, Zhan, Lou, Mingliang, Luo, Jiao, Guo, Lei, Zhang, Yu
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1511 631/1647/1513/1967 Biomedical and Life Sciences Cell Biology CRISPR-Associated Proteins - chemistry CRISPR-Associated Proteins - genetics CRISPR-Associated Proteins - metabolism Genome, Human HEK293 Cells Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Letter to the Editor Life Sciences Protein Structure, Tertiary Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics RNA - genetics RNA - metabolism Transcription Factors - genetics Transcription Factors - metabolism 人类基因组位点特异性全基因组核酸内切酶编辑技术脱靶转录激活鉴定
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Beschreibung
Zusammenfassung:	Genome editing techniques have been rapidly developing in recent decades [1]. Among them, sitespecific cleavage of genomic loci in various organisms by homing endonucleases （HEases） [2], Zinc finger nucleases （ZFNs） [3], transcription activator-like effector nucleases （TALENs） [4], and most recently the CRISPR （clustered regularly interspersed short palindromic repeats）/Cas9 system [5],
ISSN:	1001-0602 1748-7838
DOI:	10.1038/cr.2014.87