Aberrant methylation and silencing of IRF8 expression in non-small cell lung cancer

Aberrant methylation and silencing of IRF8 expression in non-small cell lung cancer

The aim of the present study was to investigate the aberrant methylation and altered expression of the interferon regulatory factor 8 (IRF8) gene in non-small cell lung cancer (NSCLC). Pyrosequencing assays were performed on 191 tumor specimens from NSCLC patients. The changes in IRF8 mRNA expressio...

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Veröffentlicht in:Oncology letters 2014-09, Vol.8 (3), p.1025-1030
Hauptverfasser: SUZUKI, MAKOTO, IKEDA, KOEI, SHIRAISHI, KENJI, EGUCHI, AYAMI, MORI, TAKESHI, YOSHIMOTO, KENTARO, SHIBATA, HIDEKATSU, ITO, TAKAAKI, BABA, YOSHIFUMI, BABA, HIDEO
Format: Artikel
Sprache:eng
Schlagworte:
Cancer
Cancer therapies
expression
Gene silencing
Genes
IRF8
Lung cancer
Lung cancer, Non-small cell
Medical prognosis
Metastasis
Methylation
Mortality
Mutation
Oncology
Oncology, Experimental
Protein expression
Proteins
pyrosequencing
Rodents
Studies
Thoracic surgery
Tumors
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Zusammenfassung:The aim of the present study was to investigate the aberrant methylation and altered expression of the interferon regulatory factor 8 (IRF8) gene in non-small cell lung cancer (NSCLC). Pyrosequencing assays were performed on 191 tumor specimens from NSCLC patients. The changes in IRF8 mRNA expression, prior to and following treatment with a demethylating agent and methylation itself, were examined in 13 lung cancer cell lines by quantitative polymerase chain reaction (qPCR) and pyrosequencing. IRF8 protein expression was examined in 94 of the 191 NSCLC specimens by immunohistochemical analysis. The IRF8 methylation level was significantly higher in the tumor tissues than in matched non-malignant lung tissues (P
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2014.2234