Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants

Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContP...

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Veröffentlicht in:	Plant signaling & behavior 2013-11, Vol.8 (11), p.e26827
Hauptverfasser:	Ledoux, Quentin, Veys, Pascal, Van Cutsem, Pierre, Mauro, Sergio, Lucaccioni, Fabio, Marko, Istvan E
Format:	Artikel
Sprache:	eng
Schlagworte:	Arabidopsis - metabolism Boron Compounds - metabolism Boronic Acids - metabolism Fluoresceins - metabolism Fluorescence fluorescence emission spectroscopy fluorescent dyes Fluorescent Dyes - metabolism hydrogen peroxide Hydrogen Peroxide - metabolism leaves microscopy Molecular Probes - metabolism Plant Leaves - metabolism Short Communication Time-Lapse Imaging
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Beschreibung
Zusammenfassung:	Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy.
ISSN:	1559-2316 1559-2324 1559-2324
DOI:	10.4161/psb.26827