Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34

The phage‐proximal part of the long tail fibres of bacteriophage T4 consists of a trimer of the 1289 amino‐acid gene product 34 (gp34). Different carboxy‐terminal parts of gp34 have been produced and crystallized. Crystals of gp34(726–1289) diffracting X‐rays to 2.9 Å resolution, crystals of gp34(781–1289) diffracting to 1.9 Å resolution and crystals of gp34(894–1289) diffracting to 3.0 and 2.0 Å resolution and belonging to different crystal forms were obtained. Native data were collected for gp34(726–1289) and gp34(894–1289), while single‐wavelength anomalous diffraction data were collected for selenomethionine‐containing gp34(781–1289) and gp34(894–1289). For the latter, high‐quality anomalous signal was obtained.