Graphene-Induced Adsorptive and Optical Artifacts During In Vitro Toxicology Assays

Graphene-Induced Adsorptive and Optical Artifacts During In Vitro Toxicology Assays

Graphene materials can deplete essential micronutrients from cell culture media and potentially interfere with probe dyes used during in vitro analysis of cell viability and function by (1) adsorbing dyes before they can interact with their target biomolecule, (2) quenching fluorescence after the in...

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Veröffentlicht in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2013-06, Vol.9 (11), p.1921-1927
Hauptverfasser: Creighton, Megan A., Rangel-Mendez, J. Rene, Huang, Jiaxing, Kane, Agnes B., Hurt, Robert H.
Format: Artikel
Sprache:eng
Schlagworte:
Absorption
adsorption
Assaying
Biotechnology
Colorimetry
Dyes
fluorescence
Graphene
In vitro testing
micronutrients
Nanotechnology
Quenching
toxicology
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Beschreibung
Zusammenfassung:Graphene materials can deplete essential micronutrients from cell culture media and potentially interfere with probe dyes used during in vitro analysis of cell viability and function by (1) adsorbing dyes before they can interact with their target biomolecule, (2) quenching fluorescence after the interaction, and/or (3) absorbing or scattering light, which are issues for both colorimetric and fluorescent assays.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201202625