Heritable/conditional genome editing in C. elegans using a CRISPR-Cas9 feeding system

Type II clustered, regularly interspaced, short palindromic repeat （CRISPR）-associated （Cas） system is a novel genome-editing tool for targeted mutagenesis in cultured cells and whole organisms . Recently, the CRISPR-Cas9 system has been applied in C. elegans using various delivery approaches including the transgene method and the direct injection of Cas9 mRNA/single guide RNA （sgRNA） or Cas9-sgRNA ribonucleoproteins . In the present study, we have developed a CRIS- PR-Cas9-based genome-editing tool for C. elegans by delivering gene-specific guide RNA （gRNA） through bacterial feeding to achieve gene disruptions in a time- and labor-saving manner.