Nanostructured substrates for isolation of circulating tumor cells

•Application of nanostructured substrates for isolation of circulating tumor cells (CTCs).•The fabrication methods for nanostructured surfaces are briefly discussed.•Underlying CTC capture mechanisms are described in detail.•We discussed major challenges faced by CTC isolation technologies.•Future directions are also discussed. Circulating tumor cells (CTCs) originate from the primary tumor mass and enter into the peripheral bloodstream. CTCs hold the key to understanding the biology of metastasis and also play a vital role in cancer diagnosis, prognosis, disease monitoring, and personalized therapy. However, CTCs are rare in blood and hard to isolate. Additionally, the viability of CTCs can easily be compromised under high shear stress while releasing them from a surface. The heterogeneity of CTCs in biomarker expression makes their isolation quite challenging; the isolation efficiency and specificity of current approaches need to be improved. Nanostructured substrates have emerged as a promising biosensing platform since they provide better isolation sensitivity at the cost of specificity for CTC isolation. This review discusses major challenges faced by CTC isolation techniques and focuses on nanostructured substrates as a platform for CTC isolation.