Estimating prion concentration in fluids and tissues by quantitative PMCA

Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samp...

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Veröffentlicht in:	Nature methods 2010-07, Vol.7 (7), p.519-520
Hauptverfasser:	Soto, Claudio, Chen, Baian, Morales, Rodrigo, Barria, Marcelo A
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1513 631/208/737/1505 631/45/470/460 692/699/375/365/1937 Amplification Animal diseases Animals Biochemical assays Bioinformatics Biological Microscopy Biological properties Biological samples Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Blood brief-communication Cricetinae Diagnosis Diagnostic specimens Enzyme-linked immunosorbent assay Hamsters Life Sciences Measurement Medical research Medicine, Experimental Mesocricetus Nervous system diseases Physiological aspects Prion diseases Prion protein Prions Protein Folding Proteins Proteomics PrPSc Proteins - blood PrPSc Proteins - urine Research methodology Scrapie Scrapie - blood Scrapie - urine Separation Spleen
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Zusammenfassung:	Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrPSc) in brain, spleen, blood and urine of scrapie-affected hamsters.
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth.1465