Gamma ray sterilization of delta inulin adjuvant particles (Advax™) makes minor, partly reversible structural changes without affecting adjuvant activity

Highlights • Advax™ adjuvant (delta inulin-based) has uniform particles ∼1–2 μm diameter. • Microparticles of this size cannot be terminally sterile-filtered. •60 Co sterilization (25 kGy) does not overtly affect adjuvant action or structure. • Isoform and reducing-end analyses show single-hit cleav...

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Veröffentlicht in:	Vaccine 2014-01, Vol.32 (5), p.552-557
Hauptverfasser:	Cooper, P.D, Barclay, T.G, Ginic-Markovic, M, Petrovsky, N
Format:	Artikel
Sprache:	eng
Schlagworte:	Adjuvant Adjuvants, Immunologic - chemistry Allergy and Immunology Animals Antigens Applied microbiology Atoms & subatomic particles Biological and medical sciences Female Fundamental and applied biological sciences. Psychology Gamma Rays Glucose Immunity Immunization Inulin Inulin - chemistry Mice Mice, Inbred BALB C Microbiology Physical properties Polymorph Protein Isoforms - chemistry Sterilization Sterilization - methods Temperature Vaccine Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Beschreibung
Zusammenfassung:	Highlights • Advax™ adjuvant (delta inulin-based) has uniform particles ∼1–2 μm diameter. • Microparticles of this size cannot be terminally sterile-filtered. •60 Co sterilization (25 kGy) does not overtly affect adjuvant action or structure. • Isoform and reducing-end analyses show single-hit cleavage of some inulin chains. • Gamma irradiation appears as a viable strategy for Advax™ terminal sterilization.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2013.11.105