Dynamic 18 F-FDG PET for Assessment of Tumor Physiology in Two Breast Carcinoma Xenografts

Purpose To compare dynamic 2-deoxy-2-[ 18 F]fluoro-D-glucose positron emission tomography ( 18 F-FDG PET) parameters in two selected human breast cancer xenografts and to evaluate associations with immunohistochemistry and histology. Procedures Dynamic 18 F-FDG PET of luminal-like MAS98.06 and basal-like MAS98.12 xenografts was performed, and the compartmental transfer rates ( k 1 , k 2 , k 3 ), blood volume fraction ( v B ) and metabolic rate of 18 F-FDG( MR FDG ) were estimated from pharmacokinetic model analysis. After sacrifice, analyses of hypoxia (pimonidazole), proliferation (Ki-67), vascularization (CD31), glucose transport receptor (GLUT1) and necrosis (HE) was performed. The level of hexokinase 2 (HK2) was estimated from Western blot analysis. Results The 18 F-FDG uptake curves for the two xenografts were significantly different ( p