Estimating telomere length from whole genome sequence data

Estimating telomere length from whole genome sequence data

Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nucleic acids research 2014-05, Vol.42 (9), p.e75-e75
Hauptverfasser: Ding, Zhihao, Mangino, Massimo, Aviv, Abraham, Spector, Tim, Durbin, Richard
Format: Artikel
Sprache:eng
Schlagworte:
Adult
Aged
Base Sequence
Cross-Sectional Studies
Exome
Genome, Human
Humans
Leukocytes - physiology
Methods Online
Middle Aged
Models, Genetic
Repetitive Sequences, Nucleic Acid
Sequence Analysis, DNA
Software
Telomere - genetics
Telomere Homeostasis
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot measurements of the mean length of terminal restriction fragments (mTRFs) and display age-dependent attrition comparably well as mTRFs.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gku181