A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as t...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2013-11, Vol.49 (88), p.10379-10381
Hauptverfasser: Acharya, Abhinav P, Nafisi, Parsa M, Gardner, Austin, Mackay, Joanna L, Kundu, Kousik, Kumar, Sanjay, Murthy, Niren
Format: Artikel
Sprache:eng
Schlagworte:
Carbocyanines - chemistry
Cell Line
Enzyme-Linked Immunosorbent Assay - methods
Fluorescent Dyes - chemistry
Horseradish Peroxidase - analysis
Limit of Detection
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Zusammenfassung:The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc44783a